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Jack Selby, managing director at Thiel Capital and founder of Copper Sky Capital, warns founders that chasing extreme valuations is a dangerous game, pointing to the painful market reset of 2022 as a cautionary tale. “If you put yourself on this high-wire act, it’s very easy to fall off,” he said.

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The first such method was developed in biophysicist Steve Quake’s lab at Caltech and commercialized by Helicos Biosciences, but became unavailable after the company declared bankruptcy, saddled by legal issues and unable to find a market niche. These days, the canonical technique comes from a company called Pacific Biosciences (PacBio). Scientists often refer to single-molecule techniques as “third-generation” DNA sequencing, though they’re often also lumped into the NGS bucket with Illumina.